THE LIQUID CHROMATOGRAPHY MASS SPECTROMETER (LC/MS) PROFILE OF GUIERA SENEGALENSIS LEAVES METHANOL EXTRACT REVEALED ITS ANTIOXIDANT POTENTIAL

Abstract

This study profiles the antioxidant potentials of methanol extract of Guiera senegalensis leaves on antioxidant activity and its LC-MS profile. Phytochemical screening for the bioactive metabolites present in the n-hexane, ethyl acetate and methanol extracts were carried out. The result shows that of all the fractions of the crude extracts, methanol extract has more of the secondary metabolites in comparison with the others, which necessitates its use in this study. The in vivo bio assays were carried out, and the analysis shows that lipid peroxidation (LPO), super oxidase dismutase (SOD) and catalase (CAT) activities have values of 66.61± 9.29, 1.53± 0.03 and 0.003± 0.001 respectively. The values of LPO and SOD are significantly different while that of CAT not significant when compared to the control and are concentration dependent. The LC-MS studies revealed the presence of some important metabolites; Tuberonic acid, Naringenin and Phenolic glycosides which are predominantly antioxidant in addition to the other molecules. Therefore, the Guiera senegalensis leaves extract contained vital antioxidants and justified its common usage in traditional medicine and can help in scavenging the effect of free radicals in both Human and animal subjects, and generally can be used in health management as immune boaster.